期刊论文

Zhang, ZJ;Wang, YS

英文

International Journal of Molecular Sciences

1661-6596

2023

24

17

13245-

10.3390/ijms241713245

Conceptualization, Z.Z.; methodology, J.Z. and Y.W.; software J.Z. and Y.W.; validation Y.W. and Z.Z.; formal analysis J.Z. and Z.Z.; investigation, Q.C.; resources, J.H. and Z.Z.; data curation, J.Z.; visualization, X.L. and Y.W.; supervision Y.L.; project administration, Z.Z.; funding acquisition, Z.Z. All authors have read and agreed to the published version of the manuscript. This work was supported by the National Natural Science Foundation of China (31672031 and 32272537), the Key Research and Development Program of Zhejiang Province, China (2021C02003), and the Key R&D Program of China (2022YFD1401204, 2022YFC2601405).

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As an endosymbiont, Wolbachia exerts significant effects on the host, including on reproduction, immunity, and metabolism. However, the study of Wolbachia in Thysanopteran insects, such as flower thrips Frankliniella intonsa, remains limited. Here, we assembled a gap-free looped genome assembly of Wolbachia strain wFI in a length of 1,463,884 bp (GC content 33.80%), using Nanopore long reads and Illumina short reads. The annotation of wFI identified a total of 1838 protein-coding genes (including 85 pseudogenes), 3 ribosomal RNAs (rRNAs), 35 transfer RNAs (tRNAs), and 1 transfer-messenger RNA ...