National Natural Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31570326]; Natural Science Foundation of Hainan Province, China [319MS084]; Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Tropical Agricultural Sciences, China [1630032019038]
机构署名：
本校为第一且通讯机构
院系归属：
园艺园林学院
摘要：
Phenylalanine ammonia lyase (PAL) is the first committed step in the formation of phenylpropanoids, and catalyses the deamination of L-phenylalanine (L-Phe) to yield cinnamic acid. While PALs are common in plants, PAL genes involved in alkaloid biosynthesis in Cephalotaxus hainanensis have never been described. To obtain better knowledge of PAL genes and their number and function involved in Cephalotaxus alkaloid biosynthesis four PAL genes were screened and cloned. In vitro enzymatic analysis showed that all four PAL recombinant proteins could...
