期刊论文

中文

生物资源

2096-3491

2015

37

1

67-70

10.14188/j.ajsh.2015.01.013

SCX1317:湖南省大学生研究性学习和创新性实验计划 2010DFA62510:国家国际科技合作专项基金

本校为第一机构

采用微生物常规分离筛选方法,以蘑菇培养基质、牛瘤胃内容物、落叶堆和腐木等为材料,从中分离筛选得到一株高产纤维素酶的真菌Z90;进一步对该菌株进行紫外诱变育种,获得产酶能力更强且遗传稳定的突变菌株Z90-19,突变菌株Z90-19产CMCase和FPA的活性分别达到11.61±0.17 U·mL-1和2.15±0.14 U· mL-1;对该菌株进行鉴定,确定菌株Z9o为核茎点霉(Phoma putaminum).

A fungus Z90 with high-yield cellulase was isolated by screening from the different conditions,such as culture medium of mashroom,rumen contents of cattle,leaf litter and decaying wood.The mutant strain Z90-19 was finally screened after treated with ultraviolet,and the CMCase and FPA activities of the strain Z90-19 reached to 11.61±0.17U·mL-1 and2.15±0.14U·mL-1,respectively.At the same time,th...