This work was financially supported by Education Commission Foundation of China for People Returning from Abroad and the Natural Science Foundation of Hunan Province.
机构署名：
本校为其他机构
院系归属：
生物科学技术学院
摘要：
Magnetic composite microspheres containing carboxyl groups could be activated by thionylchloride. The carboxy microspheres produced reactive acid chloride groups which then react with the free amino groups of enzyme to give peptide bonds (-CO-NH-). Papain was mostly immobilized on the surface of the activated magnetic carriers by covalent binding. Freundlich isothermal binding model was established, the factors involved with the relative activity and the enzymic properties of the immobilized papain were studied in comparison with its soluble co...
