Gene cloning of porcine adiponectin gene from adipose tissue and construction of its eukaryotic expression vector

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成果类型：

期刊论文

作者：

Luo, J. -J.;Song, H. -W.;Zhang, B.*;Li, L. -L.;Chen, Y. -G.;...

通讯作者：

Zhang, B.

作者机构：

[Chen, Y. -G.; Fan, J. -X.; Luo, J. -J.; Zhang, B.; Wu, L. -Z.; Zhan, J. -S.; Song, H. -W.] Hunan Agr Univ, Coll Anim Sci & Technol, Changsha 410128, Hunan, Peoples R China.

[Li, L. -L.; Fan, J. -X.] Chinese Acad Sci, Inst Subtrop Agr, Lab Anim Nutr & Human Hlth, Changsha, Hunan, Peoples R China.

[Li, L. -L.; Fan, J. -X.] Chinese Acad Sci, Inst Subtrop Agr, Key Lab Agroecol, Changsha, Hunan, Peoples R China.

[Peng, Y.] Hunan Radio & TV Univ, Fac Agr & Med, Changsha, Hunan, Peoples R China.

[Zhang, B.] Hunan Agr Univ, Coll Anim Sci & Technol, 313 Second Teaching Bldg, Changsha 410128, Hunan, Peoples R China.

通讯机构：

[Zhang, B.] H

Hunan Agr Univ, Coll Anim Sci & Technol, 313 Second Teaching Bldg, Changsha 410128, Hunan, Peoples R China.

语种：

英文

关键词：

adipocyte;recombinant;porcine;Pichia pastoris;gene expression

期刊：

JOURNAL OF ANIMAL PHYSIOLOGY AND ANIMAL NUTRITION

ISSN：

0931-2439

年：

2014

卷：

期：

页码：

517-521

DOI：

10.1111/jpn.12101

基金类别：

National Science Foundation of ChinaNational Natural Science Foundation of China (NSFC) [31072053, 30671516]; Hunan Provincial Natural Science Foundation of ChinaNatural Science Foundation of Hunan Province [11JJ2014]; Specialized Research Foundation for the Doctoral Program of Higher Education, Ministry of Education, China [20104320110001]

机构署名：

本校为第一且通讯机构

院系归属：

动物科学技术学院

摘要：

Summary: To clone adiponectin (ADPN) gene from Shaziling porcine adipocyte and construct its eukaryotic expression vector, total RNA was extracted from subcutaneous fatty tissue. One pair of specific primers was designed by Primer 5.0 software according to the sequence of ADPN gene of porcine available in GenBank. The ADPN gene was amplified by PCR from cDNA and cloned into pMD18-T vector to construct recombinant clonal vector pMD-ADPN, sequenced and analysed. A recombinant expression plasmid pPICZaA-ADPN was constructed by subcloning the clone...

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Gene cloning of porcine adiponectin gene from adipose tissue and construction of its eukaryotic expression vector

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