期刊论文

中文

植物科学学报

2095-0837

2022

40

4

533-543

10.11913/PSJ.2095-0837.2022.40533

湖南省自然科学基金（2019JJ40231）～～；

本校为第一机构

黄精属(Polygonatum)的许多物种具有重要的药用价值,但目前缺乏能够准确、高效地鉴定黄精属药用植物的DNA条形码.本研究通过对ITS、trnK-matK、rbcL、psbA-trnH和psbK-psbl序列进行扩增和测序,并从ITS序列中提取ITS2序列,共获得黄精属7个药用物种23份样品的138条序列.进一步比较6个DNA条形码对黄精属药用植物的鉴定效率,并验证所筛选条形码的可靠性.结果显示:trnK-matK的种内和种间变异重合少且有较明显的条形码间隙,其他5个序列的种内和种间变异重合多且无条形码间隙;BLAST结果表明trnK-matK的鉴定效率最高(85.7％),系统发育树显示trnK-matK的鉴定能力最强,能将全部12个多花黄精样品聚在一支,并...

Many species of the genus Polygonatum have important medicinal value,but there is currently a lack of DNA barcodes that can effectively identify medicinal plants within Polygonatum.In the present study,based on the amplification and sequencing of ITS,trnK-matK,rbcL,psbA-trnH,and psbK-psbI,and the extraction of the ITS2 sequences,138 sequences were obtained from 23 samples of seven Polygonatum medicinal species.Similarity search algorithm(BLAST)and neighbor-joining(NJ)analysis were used to compare the efficacy of sequences for identifying medicinal species and analysis of molecular variance(AMO...