期刊论文

中文

昆虫学报

0454-6296

2009

52

3

267-273

10.3321/j.issn:0454-6296.2009.03.005

基金项目：国家自然科学基金项目（30571235）

本校为第一机构

为探讨12a-羟基鱼藤酮对斜纹夜蛾 Prodenia litura(Fabricius)生殖力的影响及其作用机理,本文测定了该虫取食12a-羟基鱼藤酮后的产卵量.在用高效液相色谱仪确定其卵巢组织中存在12a-羟基鱼藤酬后,通过MTT法测定该化合物对斜纹夜蛾卵巢细胞(PL细胞)的细胞毒性,并利用流式细胞仪检测该化合物对PL细胞细胞周期、膜电位、胞内钙离子浓度、线粒体膜电位的影响.结果表明:斜纹夜蛾幼虫取食12a-羟基鱼藤酮后产卵量下降80%以上,并从卵巢组织中检测出该化合物.12a-羟基鱼藤酮对PL细胞增殖具有抑制活性,其IC_(50)为6.6 ms/L.该化合物将PL细胞周期阻滞于S期,导致PL细胞膜电位、线粒体膜电位和细胞内游离钙离...

In order to study the effect of 12a-hydroxyrotenone on the fecundity of Prodenia litura （Fabricius） and its action mechanisms, the number of eggs laid per female adult of P. litura was investigated; after 12a-hydroxyrotenone in ovary was determined with HLPC, its cytotoxicity to ovarian cells from P. litura （PL cells） was determined at 48 h after treatment by MTT assay. The effect of 12a-hydroxyrotenone on cell cycle, cell membrane potential and mitochondrial membrane potential of PL cells, and intracellular free calcium concentration in PL...