[Guan Chun-yun; Zhang Zhen-qian] Hunan Agr Univ, Oilseed Res Inst, Changsha 410128, Hunan, Peoples R China.

[Guan Chun-yun] H

Hunan Agr Univ, Oilseed Res Inst, Changsha 410128, Hunan, Peoples R China.

This paper reports a comprehensive study of screening rapeseed soil sample inVictoria blue solution solid medium;with olive oil as sole carbon source. The lipaseof Enterobacter agglomerans which has the highest activity was identified. Underthe optimum conditions determined;the enzyme activity of this strain reached 39.09 U/mL. The optimal conditions were found to be: initial pH 7.0;agitating at 30°C for 48 h;with 2.0% (w/v) lactose as carbon source;1.5% (w/v) beef extract and 1.0% (w/v) yeast extract as nitrogen source;and 0.1% (w/v) MgSO4·7H2O. Olive oil;sesame oil and tea oil as raw materials can be catalyzed to biodiesel by the lipase of this strain at 30°C and 180 rpm. And the yield reached 54.51% with sesame oil as raw material;even when they contained 92.4% (w/v) water in the starting materials. This strain will potentially serve as a promising alternative lipase for biodiesel production with raw materials containing water.

农学院