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Structure-Based Design of Porcine Circovirus Type 2 Chimeric VLPs (cVLPs) Displays Foreign Peptides on the Capsid Surface

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成果类型:
期刊论文
作者:
Wang, Dongliang;Zhang, Sujiao;Zou, Yawen;Yu, Wanting;Jiang, Yifan;...
通讯作者:
Wang, Naidong;Yang, Yi
作者机构:
[Zhan, Yang; Wang, Naidong; Wang, Dongliang; Jiang, Yifan; Zhang, Sujiao; Yu, Wanting; Zou, Yawen; Wang, ND; Yang, Yi] Hunan Agr Univ, Hunan Prov Key Lab Prot Engn Anim Vaccines, Res Ctr Reverse Vaccinol, Coll Vet Med,Lab Funct Prote, Changsha, Hunan, Peoples R China.
[Dong, Yanpeng] Jiangsu Nannong Hitech Co Ltd, Jiangyin, Peoples R China.
通讯机构:
[Wang, ND; Yang, Y] H
Hunan Agr Univ, Hunan Prov Key Lab Prot Engn Anim Vaccines, Res Ctr Reverse Vaccinol, Coll Vet Med,Lab Funct Prote, Changsha, Hunan, Peoples R China.
语种:
英文
关键词:
*3D structure prediction;*bivalent or multivalent vaccines;*capsid protein;*chimeric virus-like particles (cVLPs);*porcine circovirus type 2 (PCV2)
期刊:
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
ISSN:
2235-2988
年:
2018
卷:
8
期:
JUL
页码:
232
基金类别:
This project was supported by General Program of National Natural Science Foundation of China (Grants No. 31571432); Research Foundation of Hunan Provincial Education Department, China (Grant No. 15A086); Hunan provincial Natural Science foundation of China (Grant No.2018JJ2177); Lateral project funding (Grant No. 17430102000049 and 17430102001846); the Talent Input Project of Hunan Agricultural University (Grant No. 540490317006); and the Youth Science Foundation of Hunan Agricultural University (Grant No. 17QN10).
机构署名:
本校为第一且通讯机构
院系归属:
动物医学院
摘要:
Although porcine circovirus-like particles can function as a vector to carry foreign peptides into host cells, displaying foreign peptides on the surface of virus-like particles (VLPs) remains challenging. In this study, a plateau, consisting of the middle portion of Loop CD (MP-Lcd) from two neighboring subunits of PCV2 capsid protein (Cap), was identified as an ideal site to insert various foreign peptides or epitopes and display them on the surface of PCV2 VLPs. One of the goals of this work is to determine if the surface pattern of this pla...

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