Palisade mesophyll and spongy mesophyll of ripped middle leaves of five flue-cured tobacco varieties, K326, K346, Yun87, Yun202, HongDa, were separated by pectinase. Nonvolatile organic acid was detected by gas chromatography. Plastid pigment, glucide, and nicotine were analyzed by spectrophotometer. Results showed that the content of multiple organic acid and nicotine in spongy mesophyll was higher than that in palisade nents. In palisade 〉 Yun202; that of 〉 Yun202 〉 K346, Yun87 〉 Yun202; Yun202 〉 Yun87 〉 mesophyll, while it was on the c...