The objective of this study is to clarify the distribution characteristics of soil aggregates in different soil layers as well as its relationship with C/N in rice-tobacco rotation area of Hunan province. In 2017, 150 soil samples were collected from Chenzhou, Hengyang and Changsha of Hunan province. The size fraction, distribution characteristics and stability of soil aggregates in different soil layers were discussed. The relationship between the soil aggregate stability and C/N was studied by correlation analysis. The results indicated that ...