Cytochrome c oxidase mRNA as an internal control for detection of Potato virus Y and Potato leafroll virus from single aphids by a co-amplification RT-PCR assay

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作者信息关键词期刊信息基础信息归属信息摘要

成果类型：

期刊论文

作者：

He, Changzheng;Molen, Teresa A.;Xiong, Xingyao;Boiteau, Gilles;Nie, Xianzhou*

通讯作者：

Nie, Xianzhou

作者机构：

Agr & Agri Food Canada, Potato Res Ctr, Fredericton, NB E3B 4Z7, Canada.

Hunan Agr Univ, Fac Hort, Changsha, Hunan, Peoples R China.

[Nie, Xianzhou] Agr & Agri Food Canada, Potato Res Ctr, POB 20280,850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.

通讯机构：

[Nie, Xianzhou] A

Agr & Agri Food Canada, Potato Res Ctr, POB 20280,850 Lincoln Rd, Fredericton, NB E3B 4Z7, Canada.

语种：

英文

关键词：

Co-amplification;Cytochrome c oxidase subunit 1;Internal control;PLRV;PVY;RT-PCR;Single aphids

期刊：

Journal of Virological Methods

ISSN：

0166-0934

年：

2006

卷：

138

期：

1-2

页码：

152-159

DOI：

10.1016/j.jviromet.2006.08.007

机构署名：

本校为其他机构

院系归属：

园艺园林学院

摘要：

Using cytochrome c oxidase subunit I (COX1) mRNA as the internal control, a triplex reverse transcription-polymerase chain reaction (RTPCR) for detection of Potato virus Y (PVY) and Potato leafroll virus (PLRV) with co-amplification of COX1 from single specimens of various aphid species has been developed. Partial length cDNA of COX1 from green peach aphid, Myzus persicae (Sulzer), potato aphid, Macrosiphum euphorbiae (Thomas), buckthorn aphid, Aphis nasturtii (Kaltenbach), and pea aphid, Acyrthosiphom pisum (Harris), was cloned and sequenced. These sequences, together with existing COX1 seque...

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Cytochrome c oxidase mRNA as an internal control for detection of Potato virus Y and Potato leafroll virus from single aphids by a co-amplification RT-PCR assay

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