期刊论文

中文

水生生物学报

1000-3207

2009

33

1

89-93

10.3724/SP.J.0000.2009.10089

基金项目：科技部攻关计划（2004BA543C） 湖南省教育厅重点项目（07A012）资助

本校为第一机构

Dmrt基因家族是一个与性别决定相关的基因家族,该家族成员共有一个具有DNA结合能力的保守基序-DM结构域.为了进一步探讨该家族在系统进化中的保守性,本研究通过简并PCR技术,扩增并克隆了中国大鲵(An-drias davidianus)基因组中的DM结构域.序列分析显示,中国大鲵基因组中存在Dmrt基因的DM结构域.其核酸序列与猕猴、青鳉、人、小鼠、牛、热带爪蟾相应Dmrt基因DM结构域的相似性分别为91%、92%、92%、89%、91%、84%.其蛋白序列与上述物种的相似性均为91%,表现为4个氨基酸的变异,即第19、34、36和45位的精氨酸分别由半胱氨酸、谷胱酰胺、色氨酸和谷胱酰胺所取代.这些氨基酸的变化对其蛋白总体的三维构...

The Dmrt genes constitute a new gene family related to sex-determination, which encoded putative transcription factors with a conserved DM domain that initiates genetic process in early development through its DNA binding ability. So far, the Dmrt genes have been discovered in a wide range of animal species, such as fish, amphibian, reptiles, birds, and mammals, which evidently revealed the evolutionary conservation of Dmrt gene family. Amphibians is a key in the evolution during the aquatic and the terrestrial, the research of Dmrt gens in And...