期刊论文

中文

水生生物学报

1000-3207

2016

40

3

459-466

10.7541/2016.61

国家自然科学基金面上项目（31272652）资助~~；

本校为第一机构

实验使用RACE-PCR技术获得了赤眼鳟(Squaliobarbus curriculus)髓样分化因子88 (Myeloid differentiation factor 88, MyD88)的cDNA全长,命名为ScMyD88。ScMyD88的cDNA全长为1779 bp,其中开放阅读框855 bp, 共编码284个氨基酸残基, 推导的蛋白质分子量为33.053 kD, 理论等电点为5.66。赤眼鳟MyD88具有典型的MyD88结构特征, 包括死亡结构域和TIR结构域(Toll-IL-1 receptor domain, TIR), 其氨基酸序列和鲤科鱼类具有高度保守性, 相似性达到了90%以上, 特别是和武昌鱼相比, 相似性达到了98%。在检测的9个赤眼鳟组织和器官中均有MyD88表达, 其中肝脏、头肾和体肾中表达水平最高,在脑中表达量最低。...

The full cDNA sequence of Myeloid differentiation factor 88 （MyD88） in the barbel chub Squaliobarbus curriculus （designated as ScMyD88） has been cloned by using RACE-PCRs method. The complete sequence of ScMyD88 cDNA was 1779 bp that contained 855 bp open read frame encoding 284 amino acid residues with calculated molecular weight of 33.053 kD and a theoretical isoelectric point （PI） of 5.66. The ScMyD88 protein has typical structural features of the MyD88 family with a Death domains and a TIR domain. According to the comparison with know...