期刊论文

中文

水生生物学报

1000-3207

2016

40

5

894-901

10.7541/2016.115

国家自然科学基金面上项目（31272652） 国家自然科学基金青年项目（31402289） 湖南农业大学科学基金项目（13YJ04）资助

本校为第一机构

为探究赤眼鳟(Squaliobarbus curriculus) Toll样受体3(Toll-like receptor 3, ScTLR3)基因是否参与抗病毒免疫反应,实验运用RACE技术,克隆得到ScTLR3基因cDNA全长序列,并进行了生物信息学分析;通过Real- Time qPCR技术,检测了ScTLR3 mRNA在健康赤眼鳟10个组织中的分布以及感染草鱼呼肠孤病毒(GCRV)后肝脏、脾脏、体肾和头肾中的表达特征。结果表明: ScTLR3基因cDNA序列全长4043 bp,包括5'-非编码区(UTR) 216 bp,开放阅读框(ORF)2715 bp和3'-UTR 1112 bp; ScTLR3共编码904个氨基酸残基,推导的蛋白分子量102.67 kD,理论等电点8.76; SMART结构域预测显示, ScTLR3由N端的信号肽(SP)、富亮氨酸结构域...

To investigate whether the toll-like receptor 3 (ScTLR3) gene involve in antiviral immune response, TLR3 full-length cDNA in barbel chub (Squaliobarbus curriculus) was cloned and characterized with RACE technique and grass carp reovirus (GCRV) infection was used The method of real-time qPCR was used to examine the expression of ScTLR mRNA in 10 different tissues and the relative expression in liver, spleen, trunk kidney and, head kidney after infection at different times. The results showed that the full-length cDNA of ScTLR3 was 4043 bp including a 216 bp 5'- terminal untranslated region (UTR...