期刊论文

MOLECULAR CLONING AND EXPRESSION CHARACTERISTICS OF MX1 GENE IN SQUALIOBARBUS CURRICULUS

Xiao, T.-Y.

中文

水生生物学报

1000-3207

2020

44

4

756-763

10.7541/2020.091

基金项目: 国家自然科学基金面上项目(31572615和31272652); 中国博士后科学基金特别资助项目(2018T110833); 中国博士后科学基 金资助项目(2017M612560)资助 [Supported by the National Natural Science Foundation of China (31572615, 31272652); China Postdoctoral Science Foundation Special Funded Project (2018T110833); China Postdoctoral Science Foundation Funded Project (2017M612560)] 作者简介: 王静安(1993—), 女, 硕士; 主要研究方向为水产动物遗传育种。E-mail: 409626183@qq.com 通信作者: 陈开健, E-mail: chenkaijian2002@aliyun.com; 肖调义, E-mail: tyxiao1128@163.com

本校为第一机构

为探究赤眼鳟（Squaliobarbus curriculus）是否存在Mx1 （Myxovirus resistance）基因及参与抗病毒免疫反应,研究利用RACE技术获得了赤眼鳟Mx1基因（ScMx1）的cDNA全长序列,并对其进行了生物信息学分析;采用荧光定量PCR技术,检测了ScMx1在赤眼鳟健康组织中的表达情况以及感染GCRV后ScMx1和ScIFN-Ⅰ的表达特征。结果表明, Sc Mx1的c DNA全长为3000 bp,包含5′非编码区124 bp,开放阅读框1893 bp, 3′非编码区983 bp,共编码630个氨基酸。预测的ScMx1蛋白包含GTP酶结合区域、中央核心结构域和GTP酶效应结构域。ScMx1与青鱼Mx1的相似性最高（97%）,与ScMx的相似性仅为50%。ScMx1在所检测的10种组织中...

In order to investigate whether the Myxovirus resistance isoform (Mx1) exists in Squaliobarbus curriculus and gets involved in antiviral immune response, the full-length cDNA of ScMx1 was cloned and characterized by using RACE-PCRs technology. The expression levels of ScMx1 and ScIFN-I were detected after injection with grass carp reo-virus (GCRV) with qPCR. The full-length cDNA of ScMx1 was 3000 bp including a 5′-terminal untranslated region of 124 bp, a 3′-terminal untranslated region of 893 bp and an open reading frame of 1893 bp encoding ...