期刊论文

FULL-LENGTH cDNA CLONING AND STRUCTURAL DIFFERENCES OF THE CAST GENE FROM CTENOPHARYNGODON IDELLUS(♀), SQUALIOBARBUS CURRICULUS(♂)AND THEIR HYBRID F_1

Xiao, T.-Y.;Li, Y.-G.

中文

水生生物学报

1000-3207

2020

44

3

528-533

10.7541/2020.064

基金项目: 国家自然科学基金面上项目(31572615); 国家自然基金青年项目(31802288); 中国博士后科学基金特别资助项目 (2018T110833); 中国博士后科学基金(2017M612560); 湖南省自然科学基金(2019JJ50231); 湖南农业大学省级优秀博士学位 论文培育基金(YB2018002); 湖南省研究生科研创新项目(CX2018B400)资助 [Supported by the National Natural Science Foundation of China (31572615); the NSFC Youth Project (31802288); the Special Support Project of China Postdoctoral Science Foundation (2018T110833); the China Postdoctoral Science Foundation (2017M612560); the Natural Science Foundation of Hunan Province (2019JJ50231); Hunan Agricultural University Provincial Excellent Doctoral Dissertation Cultivation Fund (YB2018002); the Research and Innovation Project for Graduate Students in Hunan Province (CX2018B400)] 作者简介: 李东放(1996—), 男, 硕士研究生; 主要研究方向为水产遗传育种与繁殖。E-mail: dfli0927@163.com 通信作者: 李耀国(1986—), 主要研究方向为水产抗病遗传育种。E-mail: yaoguolijkl@163.com; 肖调义(1964—), 主要研究方向为水生 动物遗传育种。E-mail: tyxiao1128@163.com *共同通信作者

本校为第一机构

钙蛋白酶抑制蛋白(Calpastatin, CAST)在肌肉生长和肉质特征形成中发挥重要作用。为探究草鱼(Ctenopharyngodon idellus)(♀)×赤眼鳟(Squaliobarbus curriculus)(♂)正交F_1的肉质相关分子基础,通过RACE(Rapid-amplification of cDNA ends)技术分别克隆了草鱼(♀)×赤眼鳟(♂)F_1及其亲本的CAST基因cDNA全长,并利用生物信息学方法分析比较了三种鱼的CAST结构差异。结果表明:草鱼(♀)、赤眼鳟(♂)及草鱼(♀)×赤眼鳟(♂)F_1的CAST基因cDNA全长分别为3036、3165和3086 bp,编码901、893和904个氨基酸;预测蛋白质分子量分别为93.72、92.77和94.02 kD;推测的理论等电点分别为5.92、6.01和6.02。草鱼...

Calpastatin plays an important role in muscle growth and meat quality formation. To investigate the molecular basis of the meat quality of hybrid F1 from Ctenopharyngodon idellus (♀) × Squaliobarbus curriculus (♂), the full-length cDNAs of CiCAST, ScCAST and their hybrid F1 CAST were cloned by using RACE technology, and structure differences were analyzed by bioinformatics tools in this study. The results showed that the full-length cDNA of Ci-CAST, ScCAST and hybrid F1 CAST were 3036, 3165 and 3086 bp in length and encoded 901, 893 and 904 ...