期刊论文

Xiong, S.-T.

中文

水生生物学报

1000-3207

2022

46

2

238-247

10.7541/2021.2021.070

收稿日期: 2021-04-12; 修订日期: 2021-11-24 基金项目: 国家自然科学基金(32102782); 湖南省自然科学基金(2020JJ5244); 农业农村部热带亚热带水产资源利用与养殖重点实验室 2019年开放课题(2019A02); 湖南农业大学校科学基金(19QN04)资助 [Supported by the National Natural Science Foundation of China (32102782); Natural Science Foundation of Hunan Province (2020JJ5244); Foundation in 2019 of the Key Laboratory of Tropical and Subtropical Aquatic Resources Utilization and Aquaculture, Ministry of Agriculture and Rural Affairs of China (2019A02); Science Foundation of Hunan Agriculture University (19QN04)] 作者简介: 肖调义(1965—), 男, 博士; 主要研究方向为抗性选育与应用。E-mail: tiaoyixiao@hunau.edu.cn 通信作者: 熊舒婷(1991—), 女, 博士; 主要研究方向为抗病育种及生殖发育。E-mail: stxiong@hunau.edu.cn

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为探索基于高通量筛选抗性主效miRNA功能分子的方法应用于抗性草鱼（Ctenopharyngodon idellus）的选育,研究借助高通量测序鉴定分析感染GCRV前后的草鱼头肾组织中miRNAs。测序结果共鉴定出821个成熟miRNAs,其中118个在GCRV攻毒组特异表达, 82个为未攻毒组特有;差异分析结果显示, GCRV处理后有88个miRNA显著下调表达、46个miRNA显著上调表达; qPCR结果显示miR-21在GCRV感染组中的头肾中表达量最高, miR-194a在两个比较组中的差异倍数最大,达到48倍之高;组织表达结果发现部分差异表达miRNA在脾脏、肾脏、头肾、皮肤和鳃等免疫相关器官中高表达;这些结果提示不同的miRNAs在草鱼的GCRV应答过程中发...

Grass carp (Ctenopharyngodon idellus) which has importantly economic value is the most cultured freshwater fish in China, even in the world. The hemorrhagic disease caused by grass carp reovirus (GCRV) has greatly affected the sustainable development of grass carp aquaculture. GCRV vaccine is an important method to prevent and control hemorrhagic disease, but the multi-subtype and variation characteristics of GCRV limit the preventive effect of GCRV vaccine. Organismal immune ability is closely related to genetic factors, so many progresses hav...