期刊论文

Ji, N.;Zou, J.

中文

水生生物学报

1000-3207

2022

46

9

1293-1300

10.7541/2022.2021.0227

草鱼CD4 T辅助（Th）细胞免疫系统及其应答病毒感染的分子机理研究（32030112）资助；

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草鱼病毒性出血病的暴发严重危害草鱼养殖业的健康发展，造成巨大经济损失。草鱼呼肠孤病毒（Grass carp reovirus，GCRV）是导致该病的主要病原，本研究将Ⅰ型草鱼呼肠孤病毒GCRV-873株的外衣壳蛋白VP7基因进行原核表达，获得高度纯化VP7重组蛋白，通过免疫BALB/c小鼠，首次制备筛选得到高效价单克隆抗体。结果显示，GCRV-I vp7基因可在原核表达系统中高效表达，主要以包涵体形式存在，大小约为40 kDa。免疫小鼠后筛选到了5株IgG类型阳性杂交瘤细胞株，其中3株亚型为IgG1，2株亚型为IgG2a。Western Blotting实验和直接免疫荧光实验显示，该抗体可特异识别GCRV-873，并且ELISA检测原核重组蛋白的...

Grass carp reovirus (GCRV) poses great threat to the grass carp aquaculture industry and causes huge eco-nomic losses. Grass carp reovirus is the pathogen of this disease, which consists of three types, type Ⅰ, Ⅱ and Ⅲ. GCRV-873 is the most well studied stain of type I GCRV, with its complete genome sequenced in the 1980s. It has a segmented double stranded RNA genome with 11 fragments, encoding 7 structural proteins. VP5 and VP7 proteins form virus capsid. In this study, the capsid protein VP7 (GenBank: AF403396) of GCRV-873 strain was expr...