期刊论文

EXPRESSION CHARACTERISTICS OF SERUM PROTEIN IN GRASS CARPS SURVIVING GCRV INFECTION

中文

水生生物学报

1000-3207

2024

48

2

304-314

10.7541/2023.2023.0043

本校为第一机构

为挖掘草鱼关键抗性分子,研究利用4D label-free定量蛋白质组学技术系统分析了团队前期获得的GCRV感染存活草鱼(候选抗性草鱼)与对照草鱼的血清蛋白表达差异.共鉴定到858个草鱼血清蛋白,329个蛋白在两类草鱼中差异表达,其中163个蛋白在候选抗性草鱼血清中显著高表达,166个蛋白显著低表达.差异表达蛋白的功能主要注释为体液免疫反应调节相关蛋白,显著富集到补体凝血级联、细胞黏附和铁死亡等信号通路.对差异表达的免疫相关蛋白进行分析发现,候选抗性草鱼血清中体液免疫分子MASP2、C4a、C4b、C7b、C8b、C8g、C9、CFI、C3a.1、C3a.2、C3a.3和C3a.6等补体和抗原提呈相关免疫分子MHC1UBA和IGL4V8等蛋...

In order to explore the key resistance molecules of grass carp,we compare the serum protein expression pro-files of the candidate resistant grass carps and the control grass carps using 4D label-free quantitative proteomics.A total of 858 proteins were identified in the grass carp serum,and 329 proteins were differentially expressed in the can-didate resistant grass carps and the control grass carps,with 163 up-regulated and 166 down-regulated in the former.The differentially expressed proteins were mainly related to humoral immune response regulation,including comple-ment coagulation cascade,...