期刊论文

中文

水生生物学报

1000-3207

2024

48

2

304-314

10.7541/2023.2023.0043

National Natural Science Foundation of China, NSFC, (U20A2063) Natural Science Foundation of Hunan Province, (2021JJ40244)

本校为第一且通讯机构

为挖掘草鱼关键抗性分子,研究利用4D label-free定量蛋白质组学技术系统分析了团队前期获得的GCRV感染存活草鱼（候选抗性草鱼）与对照草鱼的血清蛋白表达差异。共鉴定到858个草鱼血清蛋白, 329个蛋白在两类草鱼中差异表达,其中163个蛋白在候选抗性草鱼血清中显著高表达, 166个蛋白显著低表达。差异表达蛋白的功能主要注释为体液免疫反应调节相关蛋白,显著富集到补体凝血级联、细胞黏附和铁死亡等信号通路。对差异表达的免疫相关蛋白进行分析发现,候选抗性草鱼血清中体液免疫分子MASP2、C4a、C4b、C7b、C8b、C8g、C9、CFI、C3a.1、C3a.2、C3a.3和C3a.6等补体和抗原提呈相关免疫分子MHC1UBA和IGL...

In order to explore the key resistance molecules of grass carp, we compare the serum protein expression profiles of the candidate resistant grass carps and the control grass carps using 4D label-free quantitative proteomics. A total of 858 proteins were identified in the grass carp serum, and 329 proteins were differentially expressed in the candidate resistant grass carps and the control grass carps, with 163 up-regulated and 166 down-regulated in the former. The differentially expressed proteins were mainly related to humoral immune response ...