Absorption and transport of myofibrillar protein-bound Nɛ-(carboxymethyl)lysine in Caco-2 cells after simulated gastrointestinal digestion

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成果类型：

期刊论文

作者：

Ye, Mengyu;Qi, Xiaomin;Ren, Xiaomin;Quan, Wei;Xu, Huaide;...

通讯作者：

Li, Mei(limei1101@nwafu.edu.cn)

作者机构：

[Ren, Xiaomin; Li, Mei; Ye, Mengyu; Qi, Xiaomin; Xu, Huaide] Northwest A&F Univ, Coll Food Sci & Engn, Yangling 712100, Peoples R China.

[Quan, Wei] Hunan Agr Univ, Coll Food Sci & Technol, Changsha 410128, Peoples R China.

[Zeng, Maomao; Chen, Jie] Jiangnan Univ, State Key Lab Food Sci & Technol, Wuxi 214122, Peoples R China.

[Chen, Jie] Jiangnan Univ, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China.

通讯机构：

[Mei Li] C

College of Food Science and Engineering, Northwest A&F University, Yangling 712100, China

语种：

英文

关键词：

Absorption;Caco-2 cell;Myofibrillar protein-bound N(ɛ)-(carboxymethyl)lysine;Simulated gastrointestinal digestion;Transport

期刊：

Food Research International

ISSN：

0963-9969

年：

2022

卷：

161

页码：

111870

DOI：

10.1016/j.foodres.2022.111870

基金类别：

This work was supported by the National Natural Science Foundation of China (Grant No. 31901788, No. 32172317); the Postdoctoral Science Foundation of China (Grant No. 2018M633586); and Doctoral Fund of Shaanxi Province (Grant No. F2020221004). The authors would like to thank the instrument shared platform of College of Food Science & Engineering of NWAFU for the assistance of Microplate Assay.

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本校为其他机构

院系归属：

食品科学技术学院

摘要：

This study aimed to investigate the absorption and transport of myofibrillar protein-bound Nɛ-(carboxymethyl)lysine (MP-bound CML) in Caco-2 cells after simulated gastrointestinal digestion. Four kinds of MP-bound CML hydrolysates with molecular weights (MWs) less than 1 kDa, 1–3 kDa, 3–5 kDa and greater than 5 kDa, were obtained by ultrafiltration; their absorption and transport were studied in Caco-2 cells. Peptide-bound CML in hydrolysates with MWs less than 1 kDa was absorbed by 6.58 % and might transport across Caco-2 cells monolayer th...

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Absorption and transport of myofibrillar protein-bound Nɛ-(carboxymethyl)lysine in Caco-2 cells after simulated gastrointestinal digestion

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