期刊论文

中文

生物技术通讯

1009-0002

2007

18

4

635-637

10.3969/j.issn.1009-0002.2007.04.024

湖南省自然科学基金项目（03JJY4006） 湖南省教育厅青年科学基金项目（04C300）

本校为第一机构

目的:用CTAB法提取皱边石杉叶片的基因组总DNA,对DNA储存于4℃的TE缓冲溶液与65℃水浴促溶2种实验处理进行为期30 d的稳定性对比实验.方法:对比分析保存于4℃的TE缓冲溶液中与65℃进行2～3次水浴促进DNA溶解释放处理的DNA的稳定性及完整性变化.结果:保存于4℃TE缓冲溶液中的DNA样品非常稳定;65℃水浴促进了DNA从包裹的多糖中溶解释放,但2～3次水浴使大片段的DNA降解.结论:DNA保存在TE缓冲溶液中非常稳定;65℃水浴尽管可提高DNA的提取量,但会使DNA发生降解.因此,一般情况下对DNA不要进行水浴促溶.

Objective： To extract genome total DNA from ftuperzia crispata fresh tissue by using CTAB method, compare DNA sample stabilities preserving in TE buffer in 4℃ with DNA samples stabilities promoting dissolve using 65℃ water bath during 30 days. Methods： Contrast DNA stabilities and integralities with storing in TE buffer in 4℃ and suffering 2-3 times 65℃ water bath. Results： DNA samples stored in TE buffer in 4℃ were very safe, 65℃ water bath could improve DNA extraction quantity but lead DNA sample to disrupt. Conclusion： DNA samples should be kept in TE buffer no...