期刊论文

Study on Phylogenetic Relationships of Boehmeria Based on TrnL-F Regions

中文

生物技术通讯

1009-0002

2011

22

1

45-48

10.3969/j.issn.1009-0002.2011.01.011

本校为其他机构

目的:探讨苎麻属植物的系统发育关系。方法:对10个种和8个变种苎麻属植物的TrnL-F序列进行PCR扩增和T/A克隆产物序列测定;选取雾水葛属的植物作为参考外类群,同样也测定其TrnL-F全序列;根据TrnL-F区碱基序列的差异计算种间的遗传距离,采用UPGMA法进行系统发育分析并得到系统发育树。结果:野线麻、福州苎麻、赤麻、悬铃叶苎麻、序叶苎麻、白面苎麻、束序苎麻、密球苎麻、疏毛水苎麻、圆叶水苎麻、灰绿水苎麻和糙叶水苎麻聚成一支;帚序苎麻、黔桂苎麻、苎麻、微绿苎麻、贴毛苎麻和青叶苎麻聚为另一支;雾水葛单独聚成一类,与苎麻属的其他种在一级分支中即分开,与其他种的亲缘关系较远。结论:帚序苎...

Objective：To study the phylogenetic relationship of Boehmeria.Methods：we amplied the sequences of TrnL-F from ten species and eight varieties of Boehmeria by PCR amplification and directly sequencing.By referring to the Pouzolzia zeylanica,its sequence of TrnL-F was set to determined too.Based on the difference in TrnL-F,the genetic distance among varieties were calculated and UPGMA tree was constructed to analyze the genetic relationships of Boehmeria.Results：The phylogenetic trees based on the data set showed that the B.japonica,B.formosan...