期刊论文

Optimization of Submerged Fermentation Medium of Agaricus blazei Murrill for Mycelial Biomass Accumulation Using Response Surface Methodology

中文

中国农业科技导报

1008-0864

2019

21

3

152-160

10.13304/j.nykjdb.2018.0456

湖南省自然科学基金项目（2018JJ2166）资助；

本校为第一机构

为了优化巴西蘑菇（Agaricus blazei Murrill）的液体发酵培养,在单因素试验基础上,以杏鲍菇（Pleurotus Eryngii）菌渣提取液（A）、酵母浸膏（B）和玉米粉（C）用量作为影响因子,巴西蘑菇的菌丝体干重（Y）为响应值,采用3因素3水平的Box-Behnken试验设计及响应面分析法对巴西蘑菇液体培养基各参数及其交互作用进行了研究。结果表明:因素A、B、C及AC的交互作用对巴西蘑菇菌丝体干重影响均达到了极显著水平,液体发酵培养基最优组分为:杏鲍菇菌渣153.5 g/L,酵母浸膏9.7 g/L,玉米粉30.5 g/L,其菌丝体生物量最大为1.785 g/100 m L,与试验模型预测值（1.796 g/100 m L）的相对误差值仅为0.62%。

In order to optimize the liquid fermentation culture of Agaricus blazei Murrill, based on single-factor experiment,taking usage of SMS extract of Pleurotus eryngii (referred to SMS) (A), yeast extract (B) and corn starch (C) as effect factors and mycelial dry weight (Y) as response value, this paper studied the parameters of liquid fermentation culture of Agaricus blazei Murrill and their interaction by 3 factors and 3 levels Box-Behnken designation combined with respond surface methodology. The results showed that parameters A, B, C and the interaction of AC on mycelial dry weight was extreme...