Object: The method mainly studies isolating theaflavins by high performance thin liquid chromatography(HPTLC) on the polyamid-thin-layer. Through three-single experiments, considering the former 7 peaks as the object, the results reveal that the solvent system is the best when methanol: acetone:acetic acid glacial equals 8:5:3 and saturate time is 20min and their Rf of the 7 peaks are 0.14,0.22,0.28,0.31,0.35,0.4,0,48 respectively.Conclusion: It has been proved to be a ...