A strain of LYSC 3 was screened by bromocresol purple media agar plate from oil rich soil. LYSC 3 was identified as Candida tropicalis by methods of morphology,physiology and molecular biology. Fermentation conditions were also optimized through single factor. Results indicated that lipase activity reached 38. 6U·mL-1 when Candida tropicalis LYSC 3 was cultured on the media of olive oil 10g·L-1,peptone 5g·L-1,sugar 20g·L-1,(NH4)2SO4 0. 5g·L-1,MgSO4·7H2O 0. 2g·L-1,K2HPO4...