期刊论文

Isolation and Identification of Antagonistic Endophytic Bacteria of Kiwi Fruit and Its Applied on Fresh-keeping Cherry Tomato Postharvest

中文

食品工业科技

1002-0306

2019

40

4

112-118

10.13386/j.issn1002-0306.2019.04.018

湖南省自然科学研究基金(2018JJ2169) 湖南省自然科学基金(2018JJ3231) 湖南农业大学2017年大学生科技创新基金(2017ZK21).

本校为第一机构

筛选具有广谱抑菌效果的内生菌并研究其发酵液对樱桃圣女果采后保鲜效果的影响。采用研磨法从健康猕猴桃的根、茎、叶和果实中分离纯化了18株内生菌,通过平板对峙法选出强活性菌株MR-1,并对其进行菌体、菌落形态的观察、生理生化测定及16S rRNA测序分析,进行菌种鉴定。进一步研究该活性菌株对8种常见致病菌的抑制效果以及樱桃圣女果主要病害菌的抑制作用。结果表明,菌株MR-1对采后致病菌拮抗效果较强,初步鉴定其为解淀粉芽孢杆菌,其对8种植物病原菌均有较强的拮抗作用,对链格孢、灰葡萄孢、辣椒炭疽病菌3种圣女果主要病害菌表现出较强的抑制作用,经内生菌MR-1发酵滤液处理的樱桃圣女果保鲜效果最...

This article aimed to screen endophytic bacteria with broad-spectrum antibacterial activity and investigate the postharvest preservation effect of their fermentation products on cherry cherries. Eighteen endophytic bacteria were isolated and purified from the roots,stems,leaves or fruits of healthy kiwifruits by grinding method. The strong active strain MR-1 was selected by plate sputum method,and the bacteria,colony morphology,physiological and biochemical determination 16 S rRNA sequencing analysis and strain identification.The inhibitory effect of the active strain on eight common pathogeni...