期刊论文

Optimization of Multi-enzymatic Extraction of Astaxanthin from Haematococcus pluvialis by Response Surface Methodology

中文

食品工业科技

1002-0306

2019

40

22

87-92

10.13386/j.issn1002-0306.2019.22.016

湖南省自然科学基金青年基金项目(2019JJ50266)。

本校为第一机构

本文研究了纤维素酶、果胶酶以及复合酶对雨生红球藻破壁效果,并通过响应面优化酶解破壁辅助提取虾青素的工艺.结果 表明,当纤维素酶和果胶酶酶活力配比1∶1 (U/U),加酶量7000 U/mL时,优化的最佳酶解条件为:酶解pH4.9,酶解温度49℃,酶解时间为6h,理论提取率为70.21％,实际提取率为71.08％±0.26％,相对误差为1.2％.综上,复合酶法辅助提取方法简单、条件温和、绿色安全、效率高,可在雨生红球藻虾青素实际提取工艺中得到应用.

In this study,the efficiency of enzymes(cellulase,pectinase and complex enzyme)on the hydrolysis of cell wall of H.pluvialis was studied.And response surface methodology was employed to optimize the hydrolysis conditions.Results indicated that when pectinase and cellulase mixed with the ratio of 1∶1(U/U),enzyme dosage 7000 U/mL,the optimal hydrolysis conditions were as follows:pH4.9,temperature 49℃,and time 6 h.Under these conditions,the theoretical and actual crude yield of astaxanthin were reached 70.21%and 71.08%±0.26%,with a relative err...