期刊论文

Extraction Optimization of Active Ingredients in Huangshan Gongju and Analysis of HPLC-MS

中文

食品工业科技

1002-0306

2020

41

22

150-156,164

10.13386/j.issn1002-0306.2020010046

湖南农业大学国家新农村发展研究院特色产业基地项目（2018xny-tc007）；

本校为第一机构

探讨黄山贡菊的抗氧化活性化学成分.以FRAP及DPPH法分析抗氧化活性,采用响应面法优化黄山贡菊抗氧化活性成分的提取条件.在乙醇浓度60％,液料比35∶1 (mL/g),超声时间25 min的条件下,黄山贡菊提取物的总抗氧化能力为(2.134±0.011) mmol/L(RSD=1.97％,n=3),与预测值(2.010 mmol/L)吻合很好.总黄酮当量为7.88 mg芦丁/L的黄山贡菊提取物的抗氧化能力相当于2.87 mmol/L亚铁离子的还原力;总黄酮当量为25.85 mg芦丁/L的提取物的DPPH自由基清除率为94.81％.采用HPLC-MS分析黄山贡菊提取物的抗氧化活性成分,共鉴定出了113个化合物,含黄酮类化合物72个,绿原酸及衍生物7个、香豆素类8个.这些化合物可能是...

The antioxidant activity and chemical composition of extracts from Huangshan Gongju was studied in this work.The extractive conditions with antioxidant activity as an indicator were optimized by response surface method.The conditions were as follows:60%ethanol aqueous,the ratio of liquid to material 35∶1(g/mL),ultrasonic time of 25 min.Under this conditions,the total antioxidant capacity of the extract was(2.134±0.011)mmol/L(RSD=1.97%,n=3),which was in good agreement with the predicted value(2.010 mmol/L).The antioxidative activity of extract...