期刊论文

Effect of gold nanoparticle on autophagy flux and exosome secretion of mesenchymal stem cells

Lian, F.

中文

上海交通大学学报. 医学版

Journal of Shanghai Jiaotong University (Medical Science)

1674-8115

2018

38

1

42-47

10.3969/j.issn.1674-8115.2018.01.008

上海交通大学医工交叉研究基金(YG2015MS51)(Shanghai Jiao Tong University Medicine-Engineering Foundation,YG2015MS51)

本校为其他机构

目的·探讨球形金纳米颗粒(AuNPs)阻断大鼠骨髓间充质干细胞(MSCs)的自噬流对外泌体分泌的影响。方法·体外培养大鼠MSCs,分别以不同浓度AuNPs(0、15、30、90、150 μg/mL)处理细胞,CCK-8法检测细胞活力,以评估AuNPs细胞毒性。细胞分3组:正常对照组、AuNPs组(20 μg/mL AuNPs作用72 h)和阳性对照组(5 μmol/L氯喹作用4 h)。透射电镜下观察超微结构,超速离心提取细胞上清液外泌体,行纳米颗粒分析仪和电镜检测。共聚焦显微镜观察细胞自噬,Western blotting验证。结果· 0~ 30 μg/mL的AuNPs对细胞增殖的影响小。AuNPs组的MSCs外泌体分泌增多,体积减小,LC3和p62表达增高(均P

Objective: To investigate the effect of gold nanoparticles (AuNPs) on exosome secretion of mesenchymal stem cells (MSCs) via blocking autophagy flux. Methods: Rat derived MSCs were treated with AuNPs (0, 15, 30, 90, 150 μg/mL), and the cell proliferation was detected with CCK-8 assay to evaluate the cytotoxicity of AuNPs. Then, MSCs were divided into normal control group, AuNPs group (20 μg/mL AuNPs for 72 h) and positive control group (5 μmol/L chloroquine for 4 h). Transmission electron microscopy was used to observe the ultrastructure of ...