期刊论文

Xie, B.

中文

生态学报

1000-0933

2010

30

14

3849-3857

国家重点基础研究发展计划资助项目（2009CB119000） 公益性行业（农业）科研专项经费资助项目（3-25 nyhyzx07-050） 国家“十一五”科技支撑计划资助项目（2006BAD07B03 2006BAD08A08 2006BAD17B08）

本校为第一机构

土壤细菌在温室土壤环境中具有十分重要的生态功能, 与温室作物以及微生物内部存在互作关系. 研究土壤细菌的群落结构组成, 有助于了解土地利用变化与生态环境效应之间的关系. 结合16S rRNA基因克隆文库和宏基因组末端测序对温室黄瓜根围土壤细菌的多样性进行了分析. 在16S文库中, 根据97%的序列相似性水平划分OTU, 共有35个OTU, 其中优势菌群是γ-Proteobacteria, 其次为Firmicutes, Bacillus为优势细菌. 在纲分类水平上, 16S文库和宏基因组末端测序结果均包含γ-Proteobacteria、α-Proteobacteria、δ-Proteobacteria、β-Proteobacteria、Actinomycetales和Firmicutes, 各纲比例有差别;在优...

Soil bacteria play an important ecological role in the ecosystem of soil in greenhouse and in the exchange of energy and substance, and have a significant inter- or intra interaction between bacterial community and greenhouse crops. Understanding of the composition of soil bacterial communities is benefit to reveal the relationship between the change of land use and the effects of entironment. In this study, total microbial genomic DNA from soil was extracted and purified by density gradient centrifugation, and 16S rRNA gene was amplified using...