Abstract The mitochondrial genome of one potamidae species Neilupotamon papileonaceum was sequenced and annotated. The mitogenome is 16,273 bp in length, containing 37 typical genes. The A + T content of the whole mitogenome is 68.9%. Most of the PCGs started with ATG and stopped with TAT, except for ATP8 and ND6 stopped with TGA, COX1, COX2, ND5, ND4, and ND4L used incomplete T as stop codon. The phylogeny tree is monophyletic among 15 related species. The relationships of N. papileonaceum, Sinopotamon yaanense, and Longpotamon yangtsekiens...