Objective To reveal the structure and diversity of oral flora in healthy people before and after chewing areca.Methods The saliva samples were collected from 8 individuals before and after chewing areca, and high-throughput sequencing was performed with metagenomic DNA.Results After 5 minutes of chewing, the relative abundances of Streptococci, Prevotella-7, Veillonella, Leptotrichia, Haemophilus, Alloprevotella, Prevotella, Porphyromonas, Treponema2, Prevotella6 and Gemella decreased significantly by 16.57%, 12.94%, 9.38%, 23.08%, 54.55%,...