期刊论文

Study on growth-promoting effect of Trichoderma asperellum M45a and its colonization in rice

中文

安徽农业大学学报

1672-352X

2021

48

02

261-265

10.13610/j.cnki.1672-352x.20210510.025

国家重点研发计划（2017YFD0200606）； 湖南农业科技创新项目（2017JC77）共同资助；

本校为第一机构

为研究棘孢木霉M45a在水稻体内的定殖规律及促生作用,利用其绿色荧光标记菌株M45a-GFP实时动态观测标记菌的定殖情况,并探究其对大田水稻的促生作用。结果表明,M45a浸种处理对水稻苗期促生效果最好,其标记菌株M45a-GFP能有效定殖于水稻根系组织。大田促生试验表明,与清水对照相比,M45a浸种处理的水稻在生长50 d时其株高、地上部干重、根际土壤总氮和硝态氮含量显著增加,分别为8.1%、10.0%、14.92%和22.34%。因此,棘孢木霉M45a具有很好的开发潜力,为木霉菌肥部分替代化肥提供了一定的理论基础。

To investigate the colonization patterns and growth-promoting effect of Trichoderma asperellum M45a in rice, we used its green fluorescence gene labelled strain M45a-GFP to observe colonization real time dynamically and explored the growth-promoting effect of M45a inoculant on rice in the field. The results showed that M45a-GFP could effectively colonize in the root tissue of rice. Compared with the control, the field growth promotion experiment showed that soaking rice seeds with M45a conidia could significantly increase the plant height, dry weight of aboveground parts, rhizosphere soil tota...