期刊论文

中文

植物保护

0529-1542

2014

40

5

80-85

10.3969/j.issn.0529-1542.2014.05.014

公益性行业（农业）科研专项（201303031）；

本校为第一机构

采用培养皿种子萌发法和盆栽法测定了湖南省境内长沙市、永州市、常德市、益阳市、浏阳市、岳阳市、娄底市7个市州10个地区油菜田看麦娘潜在抗药性种群对精喹禾灵的抗性水平，测定了常德市桃源地区看麦娘潜在抗药性种群以及敏感种群谷胱甘肽-S-转移酶（GSTs）对精喹禾灵的敏感性，对桃源地区看麦娘抗性品系及敏感品系ACCase 基因片段进行扩增和测序，比较了两种生物型的基因序列。培养皿种子萌发法测定结果表明：常德市桃源地区看麦娘抗药性生物型对精喹禾灵的抗性水平最高，抗性倍数为10．50倍，其他地区看麦娘抗性倍数在2．01～7．09倍之间，抗性水平不明显；盆栽法测定结果表明：桃源地区看麦...

In order to investigate the resistance level and mechanism of Alopecurus aequalis Sobol.to quizalofop-P-ethyl in rape,10 potential resistant biotypes were collected from 7 cities in Hunan Province and the susceptible biotype was obtained from Nanjing Agricultural University.The resistance level of A.aequalis to quizalofop-P-ethyl was assessed by two methods:seed bioassay and whole-plant bioassay.The enzymology mechanism of Taoyuan Town potential resistant biotype and Nanjing Agricultural University susceptible biotype was studied with the impor...