期刊论文

中文

植物保护

0529-1542

2019

45

1

129-134

10.16688/j.zwbh.2017475

20114320110008:高等学校博士学科点专项科研基金 11A052:湖南省教育厅科学研究项目 2016YFD0300700:国家重点研发计划 2014ZX0800102B-003:国家转基因生物新品种培育重大专项

本校为第一机构

本研究以稻曲病菌Ustilaginoidea virens黄色(非休眠)厚垣孢子、黑色(休眠)厚垣孢子、厚垣孢子萌发后产生的分生孢子(非休眠)3种孢子为材料,采用DNA甲基化敏感扩增多态性技术对供试材料基因组DNA进行甲基化分析,探究稻曲病菌休眠与非休眠厚垣孢子DNA甲基化的水平和模式。结果显示,3种形态孢子的基因组DNA甲基化水平差异明显,分生孢子基因组DNA中CCGG序列的总甲基化率为20.56%,黑色休眠厚垣孢子为25.63%,黄色非休眠厚垣孢子为33.52%。黄色和黑色的厚垣孢子主要是全甲基化模式,其全甲基化率高于半甲基化率,其中黄色厚垣孢子全甲基化率高达17.68%;分生孢子则是以半甲基化模式为主。结果对揭示稻曲病...

Methylation sensitive amplification polymorphism (MSAP) analysis was performed to profile the DNA methylation levels and pattern of three forms of dormant and non-dormant chlamydospores and conidia in Ustilaginoidea virens . The results showed that the difference of DNA methylation was obvious among them. The total methylation rate of CCGG sequence in genomic DNA of conidia was 20.56%; the black chlamydospores (dormant) was 25.63%, and the yellow chlamydospores (non-dormant) was 33.52%. The fully-methylated ratio of yellow chlamydospores was up to 17.68%, the highest among the two kinds of chl...