期刊论文

Population structure and toxigenicity profiling of Aspergillus section Nigri on the surface of grape 'Red Earth' in Lixian county

中文

植物保护

0529-1542

2020

46

6

124-130,135

10.16688/j.zwbh.2019422

国家重点研发计划(2018YFD0201300) 国家现代农业产业技术体系建设专项(CARS-29)。

本校为第一机构

葡萄和葡萄制品面临着真菌毒素的威胁,这些真菌毒素主要来自于黑色组曲霉Aspergillus section Nigri。本研究对采自湖南省澧县‘红地球’葡萄上的黑色组曲霉进行了分离鉴定及种群结构分析,并采用超高效液相色谱串联质谱技术(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)对12株代表菌株产赭曲霉毒素(ochratoxin A,OTA)和伏马毒素B 2(fumonisin B 2,FB 2)的能力进行了测定。结果表明,澧县地区‘红地球’葡萄带菌率为92%。基于钙调素序列,分离得到的131株黑色组曲霉分属于5个种,其中A.aculeatinus为优势种。12株代表菌株均不产生OTA;A.welwitschiae(4/5)及A.nig...

Grapes and grape products are threatened by mycotoxins,caused by some species of Aspergillus section Nigri.In this study,based on calmodulin gene sequencing,using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS),the Aspergillus section Nigri population and its toxigenicity in grapes in Lixian county of Hunan province were studied to confirm the food safety of table grapes.A total of 131 Aspergillus section Nigri strains were isolated from grape samples with the isolation frequency was 92%,and five species of Aspergillus section Nigri were identified,with A.aculeati...