期刊论文

Detection of expression of inserted sequence in Csu-miR260 transgenic rice

中文

植物保护

0529-1542

2021

47

06

201-207

10.16688/j.zwbh.2020429

转基因生物新品种培育重大专项（2018ZX08011-01B）； 国家自然科学基金（31772178）；

本校为第一机构

本文以具有抗二化螟性状的Csu-miR260转基因水稻为试验材料,采用TaqMan探针实时荧光定量PCR（TaqMan RT-qPCR）和茎环实时荧光定量PCR（stem-loop RT-qPCR）分别对Csu-miR260转基因抗虫水稻中Csu-miR260插入序列和剪切形成的amiR260s的表达情况进行了定量检测。发现Csu-miR260插入序列和剪切形成的20 nt和21 nt两种长度amiR260s在转基因抗虫水稻苗期的根、茎、叶中表达,且无组织表达特异性。该试验解决了人工miRNA作物中amiRNA及前体检测引物的特异性问题,为人工miRNA植物干扰序列的表达分析提供技术参考,并为miRNA转基因作物遗传表达相关安全评价提供了数据参考。

In this article,the expression of insert sequence Csu-miR260 from transgenic rice with resistance to Chilo suppressalis and two length(20 nt and 21 nt)of amiRNA formed by shearing Csu-miR260 were quantitatively determined by using TaqMan probe real-time fluorescent quantitative PCR (TaqMan RT-qPCR) and stem-loop real-time fluorescent quantitative PCR (stem-loop RT-qPCR).It was found that the expression of the insert sequence Csu-miR260 and amiRNA formed by shearing Csu-miR260 were no tissue specificity in the roots,stems and leaves of the transgenic insect-resistant rice seedlings.This test so...