期刊论文

Application of real-time fluorescence quantitative PCR technology in detection of pathogen of rapeseed clubroot in Hunan

中文

植物保护

0529-1542

2023

49

04

233-238+275

10.16688/j.zwbh.2022282

2021JJ30356:湖南省自然科学基金项目 20K071:湖南省高校科技创新平台建设经费

本校为第一机构

为完善湖南油菜根肿病的监测预警体系,分析油菜根肿病的发生与芸薹根肿菌休眠孢子量的关系,防止湖南油菜根肿病进一步蔓延。本研究依据芸薹根肿菌ITS序列设计了一对特异性引物,通过制备标准质粒构建了快速、精准的实时荧光定量PCR...展开更多 为完善湖南油菜根肿病的监测预警体系,分析油菜根肿病的发生与芸薹根肿菌休眠孢子量的关系,防止湖南油菜根肿病进一步蔓延。本研究依据芸薹根肿菌ITS序列设计了一对特异性引物,通过制备标准质粒构建了快速、精准的实时荧光定量PCR检测方法。其检测灵敏度为3×10-10μg/μL,比常规PCR高100倍;利用建立的方法检测了来自湖南各地土壤样品中芸薹根肿菌的休眠孢...

In order to improve the monitoring and early warning system of rape clubroot in Hunan,the relationship between the occurrence of rape clubroot and the amount of dormant spores of P.brassicae was analyzed,so as to prevent the further spread of rape clubroot in Hunan.In t...MORE In order to improve the monitoring and early warning system of rape clubroot in Hunan,the relationship between the occurrence of rape clubroot and the amount of dormant spores of P.brassicae was analyzed,so as to prevent the further spread of rape clubroot in Hunan.In thi...