期刊论文

中文

水产学报

1000-0615

2009

33

5

856-864

10.3321/j.issn:1000-0615.2009.05.019

湖南省科技厅攻关项目（06NK3049） 湖南省教育厅重点项目（07A012）

本校为第一机构

采用抑制性消减杂交技术构建了三角帆蚌肝脏瘟病感染期抑制性消减cDNA文库.经检验,差异表达基因均被富集了2~(10)倍左右,证明构建的cDNA消减文库具有很强的消减效率.PCR鉴定发现,在随机挑取的阳性克隆中,95%的克隆均含有0.2～1.0 kb的插入片段,这些片段可能是三角帆蚌瘟病病毒感染后差异表达基因的cDNA片段.测序共获得214个有效cDNA序列,分别属于8大类,共98个基因.其中细胞分裂基因2个、细胞结构与运动基因9个、代谢基因10个、信号传导基因7个、细胞防疫基因10个、基因与蛋白表达基因20个、未知功能蛋白基因26个,GenBank中找不到任何同源序列的基因14个.结果说明,构建的差异表达cDNA文库,可较好...

This study used suppression subtractive hybridization （SSH） for construction of the cDNA subtractive library of the liver from the living body infected Hyriopsis cumingii plague virus. After testing, differentially expressed genes were enriched in the 2^l0 times that the proof of the cDNA library has strong subtractive efficiency. Three hundred positive clones were randomly picked and identified by PCR method; 95% clones contained 0.2 - 1.0 kb by inserts, which might be the cDNA flagmenu of differentially expressed genes in Hyriopsis cumingii...