期刊论文

中文

水产学报

1000-0615

2015

39

9

1368-1379

10.11964/jfc.20150109653

现代农业产业技术体系建设专项（CARS-46-42）；

本校为第一机构

为了探讨草鱼肝胰脏脂质过量蓄积的生化基础并促进草鱼的健康养殖,采用~1H-NMR分析技术对肝脂含量分别为3%~4%和14%~16%的两组草鱼的肝胰脏和血清进行了代谢组学分析。结果表明,从草鱼肝胰脏和血清中分别检测到了58和47种代谢物。葡萄糖、别嘌呤二醇、牛磺酸、乳酸、谷氨酸、肌酸、丙氨酸、sn-甘油-3-磷酸胆碱、甘氨酸、石碳酸、次黄嘌呤、乙酸、赖氨酸、亮氨酸和缬氨酸为两组草鱼肝胰脏主要差异代谢物;其中,肝脂含量为14%~16%的草鱼肝胰脏中葡萄糖和别嘌呤二醇的含量明显高于肝脂含量为3%~4%的草鱼组,但乳酸和多种氨基酸含量则低于肝脂含量为3%~4%的草鱼组。乳酸、葡萄糖、肌酸、柠檬酸、亮氨酸...

Excessive lipid accumulation in hepatopancreas of grass carp is very common under high density and mixed cultivation. To explore the biochemical mechanism of excessive lipid accumulation in hepatopancreas and promote the healthy cultivation of grass carp,metabonomics analysis of hepatopancreas and serum of grass carp based on1H-NM R and hepatopancreas lipid were carried out. Grass carp were classified into normal hepatopancreas lipid（ 3% to 4%） group and excessive hepatopancreas lipid（ 14%-16%） group. The results indicated that 58 and 47 me...