期刊论文

Spoilage phenotypic characterization and identification of Aeromonas isolated from spoiled Larimichthys crocea

Zhu, J.

中文

水产学报

Journal of Fisheries of China

1000-0615

2017

41

6

861-869

10.11964/jfc.20160510398

国家自然科学基金(31271954);浙江省大学生科技创新计划(2016R408024);研究生科研创新基金(14020000359) National Natural Science Foundation of China(31271954);College Student's Innovation Project of Zhejiang Province(2016R408024);Innovation Project of Zhejiang Gongshang University(14020000359)

本校为其他机构

为鉴别大黄鱼腐败菌气单胞菌的致腐能力,采用生理生化和分子方法(16S rRNA)鉴定冷藏大黄鱼货架期终点不产H_2S的菌株。分析菌株在体外培养条件下的生长、胞外酶活性、三甲胺(TMA)和生物胺形成的腐败表型,将菌株接种在灭菌鱼汁中测定其致腐能力。结果显示,分离株AE03和AE04在0~37 °C及0~60的盐度下生长良好,在4和25 °C条件下AE03菌株的生长速率显著高于AE04菌株。2株细菌不产H_2S,能还原氧化三甲胺(TMAO),分解尿素和液化明胶,氧化酶、赖氨酸、鸟氨酸脱羧酶、精氨酸水解酶等都呈阳性,能利用葡萄糖。结合16S rRNA鉴定发现分离株AE03为杀鲑气单胞菌,AE04为软体动物气单胞菌。在体外培养中,2种气单...

To identify the spoilage potential of two Aeromonas strains isolated from the spoiled Larimichthys crocea, we identified the isolates that did not produce H2 S by biochemical characterization and 16 S r RNA. All of the isolates were able to grow aerobically from 4 ℃ to 37 ℃, survived below 60 salinity, and could reduce trimethylamine N-Oxide to trimethylamine（TMA）. Biochemical characterization and phylogenetic tree analysis of16 S r RNA gene showed that AE03 and AE04 belonged to A. salmonicida and A. molluscorum, repectively. The isolate AE...