期刊论文

Preliminary analysis of the structural and functional differences between Squaliobarbus curriculus and Ctenopharyngodon idella TLR19s

中文

水产学报

1000-0615

2020

44

12

1948-1959

10.11964/jfc.20200112139

国家自然科学基金面上项目（31972787）； 国家自然基金青年项目（31802288）~~；

本校为第一机构

为探索赤眼鳟TLR19（Sc TLR19）与已报道草鱼TLR19（Ci TLR19）的结构及功能差异,实验克隆了Sc TLR19基因全长并分析其结构特点,利用实时荧光定量PCR（qPCR）分析其组织表达及草鱼呼肠孤病毒（GCRV）感染后的表达特征,同时采取过表达实验揭示其对TLR19信号通路下游功能基因的激活特性。结果显示,Sc TLR19基因编码957个氨基酸,与Ci TLR19氨基酸序列具有高度相似性（94.04%）;但Sc TLR19胞外区由9个亮氨酸重复序列（LRR）组成,比Ci TLR19在654～677氨基酸位点之间明显多1个LRR,且在三级结构上相应多2个α-螺旋和6个β-折叠。Sc TLR19广泛表达于赤眼鳟各组织,GCRV攻毒后,其表达水平在免疫组织肾脏中...

In order to explore the structural and functional differences between a TLR19 homolog(designated as ScTLR19)from S.curriculus and the reported TLR19(designated as CiTLR19)from C.idellus,we cloned the fulllength cDNA sequence of ScTLR19 gene and analyzed its sequence and structural characteristics,investigated its expression patterns of various tissues and the responses to grass carp reovirus(GCRV)infection with real-time fluorescent quantitative PCR(qPCR)technique,and revealed its distinct regulation on the downstream functional genes of TLR19 ...