期刊论文

Advances of immune-related microRNA research in teleost fish

中文

水产学报

1000-0615

2021

45

08

1430-1437

10.11964/jfc.20200312202

31972787:国家自然科学基金 SYL201802020:湖南农业大学双一流建设专项

本校为第一机构

MicroRNA （miRNA）是一类长度为18～25 nt的非编码RNA,在硬骨鱼免疫应答中起重要作用。近年来,借助高通量测序与荧光定量PCR （RT-PCR）技术,筛选到大量免疫相关的差异表达miRNA （DE miRNA）。通过比较多种硬骨鱼在不同病毒/细菌感染条件的miRNA表达谱,筛选出相同DE miRNA,并认为筛选出的DE miRNA是潜在参与调节宿主免疫应答的进化上保守的候选DE miRNA。据此,我们在病毒感染表达谱中筛选到10个进化保守的DE miRNA,细菌感染表达谱中筛选到8个保守的DE miRNA。此外,miRNA的功能研究也取得较大进展,宿主miRNA不仅可直接抑制病毒的复制,同时参与调控干扰素反应、炎症、细胞凋亡等过程。本文简述了...

MicroRNAs,which are small non-coding RNAs with 18-25 nt,play important roles in immune response of teleost fish.Recently,more and more differentially expressed miRNAs(DE miRNAs)associated with immune response have been identified through high-throughput sequencing and RT-PCR technology.It is assumed that if the same miRNAs are detected as DE miRNAs in several species of teleost fish challenged with different viruses/bacteria,they are likely to be evolutionarily conserved miRNAs involved in the regulation of immune gene networks in the hosts.Based on the above assumption,ten miRNAs were discove...