期刊论文

Transgenetic expression coat protein of Chinese wheat mosaic virus (CWMV) enhances resistance of Nicotiana benthamiana to CWMV

中文

浙江农林大学学报

2095-0756

2020

37

02

291-295

10.11833/j.issn.2095-0756.2020.02.013

CARS-3-1:现代农业产业技术体系 2016ZX08002001:国家科技重大专项

本校为第一机构

【目的】中国小麦花叶病毒（Chinese wheat mosaic virus, CWMV）是引起小麦黄花叶病的重要病原之一。获得表达CWMV外壳蛋白（coat protein, CP）的转基因烟草Nicotiana benthamiana（OECP）并分析其抗病性,为培育小麦抗病材料奠定工作基础。【方法】利用体外重组DNA技术构建含有CWMV外壳蛋白基因的表达载体,并通过农杆菌介导的转化方法,获得OECP。进一步利用Western Blot和PCR检测明确CWMV的外壳蛋白在OECP中能够正常的表达。【结果】部分转基因阳性植株出现矮化表型。此外,OECP阳性植株接种CWMV后7 d的定量分析表明,OECP中CWMV运动蛋白的表达量显著受到抑制。【结论】在烟草中表达CWMV的CP蛋白...

[ Objective ]Chinese wheat mosaic virus (CWMV) is an important virus causing wheat mosaic virus disease. The objective of this study is to obtain expression transgenic tobacco (OECP) of CWMV coat protein (CP) gene and analyze its disease resistance, which lays a foundation for the cultivation of wheat disease resistance materials. [Method]The expression vector containing the coat protein gene of CWMV was constructed by in vitro recombinant DNA technology and OECP was obtained by Agrobacterium-mediated transformation assay. Western Blot and PCR analysis showed that the CP gene of CWMV was corre...