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The Comparison of PCR Kits for the Detection of Erythrocytic Parasites on Filter Paper

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成果类型:
期刊论文
作者:
Tao, Zhi-yong;Zhang, Pei-yi;Zhang, Lu;Li, Chun-cao;Hu, Rui;...
通讯作者:
Tao, Z.-Y.
作者机构:
[Tao, Zhi-yong; Hu, Rui; Cao, Yu-jie; Li, Chun-cao; Fang, Qiang; Xia, Hui; Zhang, Pei-yi; Hua, Xiu-min; Zhang, Lu; Ni, Yi-xuan; Jin, Xiao-xia] Bengbu Med Coll, Dept Microbiol & Parasitol, 2600 Donghai Ave, Bengbu 233030, Peoples R China.
[Tao, Zhi-yong; Hu, Rui; Cao, Yu-jie; Li, Chun-cao; Fang, Qiang; Zheng, Chen-chen; Wang, Dao-jin; Xia, Hui; Zhang, Pei-yi; Hua, Xiu-min; Zhang, Lu; Ni, Yi-xuan; Jin, Xiao-xia; Fang, Run-qi] Bengbu Med Coll, Anhui Key Lab Infect & Immunol, 2600 Donghai Ave, Bengbu 233030, Peoples R China.
[Zhu, Han-wu] Chenzhou Ctr Dis Control & Prevent, Chenzhou 423000, Hunan, Peoples R China.
[Zhou, Bei] Hunan Agr Univ, Coll Vet Med, Changsha 410128, Hunan, Peoples R China.
[Wu, Kai] Wuhan Ctr Dis Control & Prevent, Wuhan 430022, Hubei, Peoples R China.
通讯机构:
[Chun-cao Li; Yu-jie Cao; Pei-yi Zhang; Zhi-yong Tao; Lu Zhang; Rui Hu] D
[Bei Zhou; Ling-xu Li; Da-wei Yao; Han-wu Zhu] C
[Dao-jin Wang et al.] A
[Kai Wu] W
College of Veterinary Medicine,Nanjing Agricultural University,No. 1 Weigang Road,China<&wdkj&>Wuhan Center for Disease Control and Prevention,Wuhan 430022,Hubei,China<&wdkj&>Department of Microbiology and Parasitology,Bengbu Medical College,2600 Donghai Avenue,China<&wdkj&>Anhui Key Laboratory of Infection and Immunology,Bengbu Medical College,2600 Donghai Avenue,China<&wdkj&>College of Veterinary Medicine,Hunan Agricultural University,Changsha 410128,China<&wdkj&>Chenzhou Center for Disease Control and Prevention,Chenzhou 423000,Hunan,China<&wdkj&>Anhui Key Laboratory of Infection and Immunology,Bengbu Medical College,2600 Donghai Avenue,China
语种:
英文
关键词:
Introduction;Materials and Methods;Results;Discussion;Conclusion;Abstract;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interests;Authors’ Contributions;Funding Statement;Acknowledgements;Acknowledgments;Supplementary Materials;Reference;Dataset Description;Dataset Files;Abstract;Introduction;Introduction and Materials;Introduction and Methods;Materials;Materials and Methods;Methods;Results;Discussion;Results and Discussion;Discussion and Conclusion;Results and Conclusion;Conclusion;Conclusions;Data Availability;Additional Points;Ethical Approval;Consent;Disclosure;Conflicts of Interest;Authors’ Contributions;Funding Statement;Acknowledgements;Supplementary Materials;References;Appendix;Abbreviations;Preliminaries;Introduction and Preliminaries;Notation;Proof of Theorem;Proofs;Analysis of Results;Examples;Numerical Example;Applications;Numerical Simulation;Model;Model Formulation;Systematic Palaeontology;Nomenclatural Acts;Taxonomic Implications;Experimental;Synthesis;Overview;Characterization;Background;Experimental;Theories;Calculations;Model Verification;Model Implementation;Geographic location;Study Area;Geological setting;Data Collection;Field Testing;Data and Sampling;Dataset;Literature Review;Related Works;Related Work;System Model;Methods and Data;Experimental Results;Results and Analysis;Evaluation;Implementation;Case Presentation;Case Report;Search Terms;Case Description;Case Series;Background;Limitations;Additional Points;Case;Case 1;Case 2 etc.;Concern Details;Retraction Details;Copyright;Related Articles
期刊:
Journal of Tropical Medicine
ISSN:
1687-9686
年:
2022
卷:
2022
基金类别:
Key Joint projects of Bengbu City and Bengbu Medical College [BYLK201828]; National and Anhui Provincial College Student's Innovation and Entrepreneurship Training Program [202110367025, S202010367023]; Scientific Innovation Project for Graduate Student of Bengbu Medical College [Byycxz20004]; Key Program and the Major Project of Natural Science Project of Anhui Higher Education Institutions [KJ2020A0567, KJ2021ZD0077]
机构署名:
本校为其他机构
院系归属:
动物医学院
摘要:
Dried blood spot (DBS) based PCR was considered an inexpensive and feasible method for detecting pathogens in the blood. The DBS carrier filter paper and PCR kits are crucial for accurate diagnosis. We evaluated 4 types of filter papers and 20 PCR kits for DBS samples. The PCR detecting Plasmodium results showed that the minimum detection limit of the 4 filter papers was 1 × 102 parasites/μL, and the positive rates of 20 PCR kits ranged from 0% to 100%. PCR results were satisfactory for detecting Plasmodium falciparum (P. falciparum) and Plas...

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