National Natural Science Foundation of China [32250710146, 32281340018, 31872027]; China Agriculture Research System [CARS-23-C08]; China Postdoctoral Science Foundation [2022M721111]
机构署名：
本校为第一且通讯机构
院系归属：
植物保护学院
摘要：
A stable and specific heat shock protein 27.2 (HSP27.2) antibody was prepared and analyzed for protein level research in Helicoverpa armigera (Hubner) (Lepidoptera: Noctuidae). The full-length hsp27.2 was amplified from H. armigera larvae and constructed into the prokaryotic expression vector. The purified His-tag fused protein was used to immunize rabbits for the antibody preparation. Western blot analysis indicated that this antibody specifically recognized the HSP27.2 encoded by H. armigera and detected the HSP27.2 encoded by other noctuid larvae. Further analysis of HSP27.2 expression in H...
