期刊论文

Huang, S.;Yan, J.

英文

NATURE PLANTS

2055-026X

2021

7

8

1026-1036

10.1038/s41477-021-00963-5

We thank T. Liu (Wuhan University) for sharing plasmids that were used for the preparation of taxadiene in E. coli. We thank Y. Zhang (Shanghai University) and S. Cheng (Agricultural Genomics Institute at Shenzhen) for helpful discussion. We thank T. Feng (Wuhan Botanical Garden, Chinese Academy of Sciences) for valuable help with TS evolution. This work was supported by the National Key R&D Program of China (grant nos 2018YFA0903200, 2018YFA0901800 and 2020YFA0907900), Research Funds for Central Nonprofit Scientific Institution (grant no. Y2020XK23), the Elite Young Scientists Program of CAAS, the Agricultural Science and Technology Innovation Program, National Science and Technology Basic Special Project (grant no. 2017FY100100), the Scientific Research Fund of Hunan Provincial Education Department (grant no. 2016XYX001), Double First-Class Construction Project of Hunan Agricultural University (grant no. SYL201802026), Fund of the Education Department of Hunan Province (grant no. 18B124) and the National Natural Science Foundation of China (grant no. 32000236).

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The ancient gymnosperm genus Taxus is the exclusive source of the anticancer drug paclitaxel, yet no reference genome sequences are available for comprehensively elucidating the paclitaxel biosynthesis pathway. We have completed a chromosome-level genome of Taxus chinensis var. mairei with a total length of 10.23 gigabases. Taxus shared an ancestral whole-genome duplication with the coniferophyte lineage and underwent distinct transposon evolution. We discovered a unique physical and functional grouping of CYP725As (cytochrome P450) in the Taxus genome for paclitaxel biosynthesis. We also iden...