Plant often subject to many biotic and abiotic factors in plant growth and development phases, and it must adapt to the environment through regulation of genes. Transcription factors （TFs） play important roles in plant development and its response to the environment, so we need to research the function of transcription factors deeply. As a result, a variety of reverse genetics tools have been developed to study TF function, include antisense suppression, overexpression, knockout and so on. The two most commonly used ones are knockout and over...
