期刊论文

中文

分子植物育种

1672-416X

2016

14

6

1455-1461

10.13271/j.mpb.014.001455

(2015CB150200):本研究由国家重点基础研究发展计划(973)项目 (2012AA101107-3):国家高技术研究发展计划(863计划)项目

本校为第一机构

植物溶血磷脂酸酰基转移酶(lysophospholipid acid acyltansferase,LPAT)是在植物不同组织中调控溶血磷脂酸生成各种磷脂酸的代谢流的关键酶。本研究采用同源克隆的方法,从甘蓝型油菜湘油15中克隆 BnLPAT4基因翻译起始位点上游的调控序列,长度为1 326 bp。Plantcare在线分析表明:该序列含有 CAAT-box和TATA-box等核心启动子原件,同时还有多个光响应元件、逆境胁迫响应元件、激素应答元件等。将该启动子与GUS基因融合,构建pBnLPAT4: GUS 植物表达载体,通过农杆菌介导法获得拟南芥T3代转基因株系。GUS组织化学染色显示,幼苗期的转基因拟南芥在叶和根部均具有GUS 活性,成熟期在莲座叶、花瓣、花萼、...

Lysophosphatidyl acyltransferase (LPAT) is an essential enzyme controlling the metabolic flow of lysophosphatidic acid into different phosphatidic acids in diverse tissues of the plants. In this study, we isolated a 1 326 bp upstream regulation sequence of BnLPAT4 gene from Brassica napus Xiangyou15 by Homology-based cloning method. Plantcare online analysis showed that the BnLPAT4 promoter region contained some typical cis-elements of eukaryotes, such as TATA-box, CAAT-box and involved multiply light-response elements, stress-induced elements ...